Inside the Black Box: What Makes SELEX Better?

Inside the Black Box: What Makes SELEX Better?

Blog Article

Aptamers are small oligonucleotides that are capable of binding specifically to a target, with impressive potential for analysis, diagnostics, and therapeutics applications.Aptamers are isolated from Outdoor Ottoman with Cushion large nucleic acid combinatorial libraries using an iterative selection process called SELEX (Systematic Evolution of Ligands by EXponential enrichment).Since being implemented 30 years ago, the SELEX protocol has undergone many modifications and improvements, but it remains a laborious, time-consuming, and English Girth costly method, and the results are not always successful.Each step in the aptamer selection protocol can influence its results.This review discusses key technical points of the SELEX procedure and their influence on the outcome of aptamer selection.